HIV-1 Detection by Western Blot Lab Report Example | Topics and Well Written Essays - 1500 words - 1

HIV-1 Detection by Western Blot - Lab Report Example The antibody when exposed to the enzyme with the substrate will lead to the production of a band that is colored (Luttmann, Bratke, Kupper and Myrtek, 2006). The test is done several times in the positive and the negative control serum to bring the clear identification of the viral protein. In the carrying out the test, there is the consideration of the nine HIV specific bands. When the bands are exposed to the serum of the individual the reaction that occurs in the bands and the patterns is what results to the diagnosis of the individual with the HIV virus. In the experiment to determine the availability of the virus, the polyacrylamide gel is extracted from the tray and the nylon that is used in the experiment is place directly onto the gel. After the nylon membrane is placed on the gel, bands of protein are transferred to the surface of the nylon membrane which makes them to be absorbed by the nylon membrane through the hydrophobic bonds (Wikipedia, 2013). The transfer between the bonds is achieved in chambers that are specially designed through capillary flow or by application of vacuum. DNA and RNA microarrays have been used in the determination of the infection with HIV since they are the key ways in the understanding of the expression of the genes and the method also allow for the rapid quantification of many of the genes once in the given cell that is focused. The microarrays have focused on the determination of the effect of the HIV on the single viral proteins in the individual in the line of lymphoid cells. The microarrays are used in the determination of the HIV due to the instance that the expression of the genes in an individual are always altered during the time of infection with the HIV (Luttmann, Bratke, Kupper and Myrtek, 2006). Infection in the individual is confirmed when the microarrays multiplex leading to the detection and also differentiate the sources that has brought the infection in the individual. In the carrying out of